Author Archives: NateKrefman

Journal Club on Monday, June 3

For our Journal Club on June 3, Daphné Dambournet presented the following paper:

Divergent modes for cargo-mediated control of clathrin-coated pit dynamics. Soohoo AL, Puthenveedu MA. Mol Biol Cell. 2013 Jun;24(11):1725-34. PMID: 23536704.

Soohoo MBoC 2013

Microtubules and Mitosis Journal Club on Thursday, May 23

For Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab discussed the following paper, selected by Anthony Cormier:

Tension sensing by Aurora B kinase is independent of survivin-based centromere localization. Campbell CS, Desai A. Nature. 2013 May 2;497(7447):118-21. PMID: 23604256.

Campbell Nature 2013

Journal Club on Monday, May 13

For our Journal Club on May 13, Christa Cortesio presented the following paper:

ER-to-plasma membrane tethering proteins regulate cell signaling and ER morphology. Manford AG, Stefan CJ, Yuan HL, Macgurn JA, Emr SD. Dev Cell. 2012 Dec 11;23(6):1129-40. PMID: 23237950.

 Manford et al Dev Cell 2012

Journal Club on Monday, April 29

For our Journal Club on April 29, Sun Hae Hong presented the following paper:

The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Miller SE, Sahlender DA, Graham SC, Höning S, Robinson MS, Peden AA, Owen DJ. Cell. 2011 Nov 23;147(5):1118-31. PMID: 22118466

Microtubules and Mitosis Journal Club on Thursday, April 25

For our new Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab discussed the following paper, selected by Nate Krefman:

Estimating the microtubule GTP cap size in vivo. Seetapun D, Castle BT, McIntyre AJ, Tran PT, Odde DJ. Curr Biol. 2012 Sep 25;22(18):1681-7. PMID: 22902755

Journal Club on Thursday, April 18

For our Journal Club on April 18, Akemi Kunibe presented the following paper:

Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Lackner LL, Ping H, Graef M, Murley A, Nunnari J.  Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):E458-67. PMID: 23341591.

Journal Club on Monday, March 17

For our Journal Club on March 17, Rebecca Lu will present the following paper:

Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism. Jose M, Tollis S, Nair D, Sibarita JB, McCusker D. J Cell Biol. 2013 Feb 18;200(4):407-18. PMID: 23401000

37 Degrees perform “I Want to Graduate” live at MCB Follies!

Synthetic boy band 37 Degrees made a surprise live performance at MCB Follies this year.  Before a screaming hoard of desperate graduate students, 37 Degrees performed their new single “I Want to Graduate.”  You may recognize the song as a parody of the Backstreet Boys single “I Want it That Way.”

You may also recognize one of the members of 37 Degrees as Nate Krefman, a graduate student in the Drubin/Barnes Lab!  The other participants in this transient complex are fellow MCB graduate students Phil Cleves and Andrew Glazer from Craig Miller’s Lab (also from “I’m Bringin’ Stickleback”), Alec Sexton from Cathy Collins’ Lab (also from “Grad School Pressure”), and Brock Roberts from Henk Roelink’s Lab (also from “Vol II: How to Do a DNA Mini-Prep (Molecular and Cell Biology Training Video Series”).


37 DEGREES is Nate Krefman, Phil Cleves, Andrew Glazer, Alec Sexton, & Brock Roberts

LYRICS: Meru S. & Nate Krefman
LIVE VOCALS: 37 Degrees
CAMERA: David McCleary



It is my fire —
my one desire.
Believe when I say:
I want to graduate.

But we are two worlds apart,
Just years from the start.
It’s your fate:
You’ll never graduate.

Tell me when…
I’m begging my committee.
Tell me when…
Be in grad school till I’m fifty.
Tell me when…
You never wanna hear me say:
I want to graduate

It will be so wrong,
If this just goes on.
Please don’t come too late.
Cuz I want to graduate.

Tell me when…
All my friends are wearing Polo.
Tell me when…
But I’m still dressing like a hobo.
Tell me when…
You never wanna hear me say:
I want to graduate.

Now I can see that you wanna depart —
But not if it’s up to me. Yeah.
No matter your progress,
I want you to know,
There’s no way you’ll ever leave.

It is my fire —
My one desire.

You never hear me!

I’m ready for my thesis.
I could even do a defense.
I never wanna hear you say:
You’ll never graduate.

Tell me when…
This stipend ain’t a salary.
Tell me when…
I wanna get better pay.
Tell me when…
You never wanna hear me say:
I want to graduate.

Cuz I want to graduate…

Life here in the UC is much better than anything they got out there!

Members of the Drubin/Barnes Lab and others collaborated this month to create musical magic (or madness?) in the form a parody of the musical number “Under the Sea” from Disney’s The Little Mermaid.  The video was a submission for UC-Berkeley Department of Molecular and Cell Biology‘s annual funny movie event MCB Follies.

We hope you enjoy it!

CONCEPT: Akemi Kunibe

LYRICS: Eric Lewellyn

VOCALS: Nathaniel Krefman

BACKING VOCALS: Sun Hae Hong & Akemi Kunibe

CINEMATOGRAPHY: Akemi Kunibe, Jeff Woodruff, & Eric Lewellyn

EDITING: Jasper Weinberg & Akemi Kunibe

STARRING: Eric Lewellyn, Rebecca Lu, & Jasper Weinberg

CAST (in order of appearance): Rebecca Lu, Jasper Weinberg, Eric Lewellyn, Jeff Woodruff, Akemi Kunibe, Kathryn Sievert, Adrienne Greene, Sun Hae hong, Benjamin Gowen, Wendy Ingram, Kevin Barry, Caitlin DeJong, aileen Kelly, Caitlin Shartner, Christopher Alvaro, Jennifer Cisson, Nathaniel Krefman, Colin Cerretani, Thomas Burke, Julia Durack, Christina Glazier, & Michelle Reniere



Private industry, it’s a mess. Life here in the UC Berkeley is much better than anything they got out there.

The money is always greener,
In some private company.
You quit you could be a tech there,
But you would be sad you’ll see.
Just look at the world around you,
Right here through the lab room door.
Such wonderful things surround you.
What more is you lookin’ for?

In the UC!
In the UC!
Darling it’s better,
Grab your pipetter,
Come follow me!
Here you can surf for half the day.
With a real job you’ll slave away.
Ya we be chillin’.
Time we be killin’.
In the UC!

Right here all the postdoc happy.
They follow where research go.
The industry doc ain’t happy —
They feel like they sold their soul.
But doc with a job is lucky —
They in for a worser fate:
One day when the boss sell company,
Guess who eatin’ ramen plate!
Uh Oh!

In the UC!
In The UC!
We doin’ science.
We not some appliance,
for makin’ money.
They got a team of robots manned.
We got the Zen of workin’ by hand.
We got no worries,
Troubles or hurries,
In the UC!
In the UC!
Each protein gel here,
Runnin’ so well here,
Bands are so clean.
Even though takin’ lots of class,
Can be a real pain in the ass,
While we be learnin’,
money be earning,
In the UC!

The tub is the bass,
the drum is the flask,
the beakers are good,
for most any task.
Our science is play.
We do every day,
Like Nate, who’s the Duke of Clone.
He put DNA,
In cells on the plate.
The sequence is good.
There ain’t no debate.
Right here where it’s at,
Our microscope phat.
An’ oh that protein glow!

In the UC!
In the UC!
No scientist here,
Like to get pissed here,
You will agree.
We got a campus full a perks.
They got a boardroom full of rich jerks.
Each undergrad here,
Learning like mad here.
In the UC!
None of our tech here,
is pain in the neck here.
In the UC!
Each PI swell here.
Get the Nobel here.
Publish in Science,
Nature, and Cell here.
Stay academic.
The fun is pandemic!
In the UC!

Journal Club on Monday, Feb. 7

For our Journal Club on February 7, Yansong Miao will present the following paper:

Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging. Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL. Science. 2012 Jun 1;336(6085):1164-8. doi: 10.1126/science.1218062. PMID: 22654058