The Drubin/Barnes Lab is interested in the molecular mechanisms that underlie highly dynamic actin-mediated membrane trafficking events. These studies are being carried out in mammalian cells and in budding yeast. The approaches employed for these studies include state-of-the-art real-time image analysis of live cells, genome-wide functional analyses, genetics, molecular genetics, and biochemistry.
Another aspect of our research concerns the molecular mechanisms that control the fidelity of mitosis and meiosis. By employing proteomics, genetics, cellular imaging, and biochemistry, we have identified novel protein components of yeast spindles and kinetochores, and have mapped their phosphorylation sites, allowing us to perform genetic tests of the importance of these proteins and their modification by protein phosphorylation.