* research articles
# reviews & methods
^ commentary


Bergman, Z. J., Wong, J., Drubin, D. G., Barnes, G. (2023). Cik1 and Vik1 Accessory Proteins Confer Distinct Functions to the Kinesin-14, Kar3  JCB (2023),

Stoops, E. H.*, Ferrin, M. A.*, Jorgens, D.M, Drubin, D. G. (2023). Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers  PNAS (2023), * co-first

Torvi, J. R*., Wong, J*., Serwas, D, Moayed, A, Drubin, D. G., Barnes, G. (2022). Reconstitution of kinetochore motility and microtubule dynamics reveals a role for a kinesin-8 in establishing end-on attachments eLife (2022), *co-first

Jin, M., Shirazinejad, C. R., Wang, B., Yan, A., Schöneberg, J., Upadhyayula, S., Xu, K., Drubin, D. G. (2022). Branched actin networks are organized for asymmetric force production during clathrin-mediated endocytosis in mammalian cells Nat Comms (2022), Article number: 3578

Cail, R. C., Shirazinejad, C. R., Drubin, D. G. (2022). Induced nanoscale membrane curvature bypasses the essential endocytic function of clathrin J Cell Biol (2022) 221 (7): e202109013.

Serwas, D., Akamatsu, M., Moayed, A., Vegesna, K., Vasan, R., Hill, J. H., Schöneberg, J., Davies, K., Rangamani, P., Drubin, D. G. (2022). Mechanistic insights into actin force generation during vesicle formation from cryo-electron tomography Dev Cell j.devcel.2022.04.012.

Kaplan, C., Kenny, S. J., Chen, X., Schöneberg, J., Sitarska, E., Diz-Muñoz, A., Akamatsu, M., Xu, K., Drubin, D. G. (2022). Load adaptation by endocytic actin networks MBOC mbc. E21-11-0589.


* Lu, M., Drubin, D. G. (2021). Unexplored Cdc42 functions at the budding yeast nucleus suggested by subcellular localization Small GTPases

* Li, K. W., Lu, M. S., Iwamoto, Y., Drubin, D. G., Pedersen, R. T. A. (2021). A preferred sequence for organelle inheritance during polarized cell growth. J Cell Science 2021, 134 (21): jcs258856.

* Chan, K. M. C., Arthur, A. L., Morstein, J., Jin, M., Bhat, A., Schleinger, D., Son, S., Stevens, D. A., Drubin, D. G., Fletcher, D. A. (2021). Evolutionarily related small viral fusogens hijack distinct but modular actin nucleation pathways to drive cell-cell fusion. PNAS 2021, 118 (1) e2007526118.


* Pedersen, R. T., Hassinger, J. E., Marchando, P., Drubin, D. G. (2020). Spatial Regulation of Clathrin-Mediated Endocytosis through Position-Dependent Site Maturation. J Cell Biol 2020, 219 (11), jcb.202002160.

* Lu, M. S., Drubin, D. G. (2020). Cdc42 GTPase Regulates ESCRTs in Nuclear Envelope Sealing and ER Remodeling. J Cell Biol 2020, 219 (8), jcb.201910119.

* Gómez-Varela, A. I., Stamov, D. R., Miranda, A., Alves, R., Barata-Antunes, C., Dambournet, D., Drubin, D. G., Paiva, S., De Beule, P. A. A. (2020). Simultaneous Co-Localized Super-Resolution Fluorescence Microscopy and Atomic Force Microscopy: Combined SIM and AFM Platform for the Life Sciences. Scientific Reports, 10 (1), 1122, s41598-020-57885-z.

* Akamatsu, M., Vasan, R., Serwas, D., Ferrin, M. A., Rangamani, P., & Drubin, D. G. (2020). Principles of self-organization and load adaptation by the actin cytoskeleton during clathrin-mediated endocytosis. eLife, 9, e49840.


* Sun, Y., Schöneberg, J., Chen, X., Jiang, T., Kaplan, C., Xu, K., Pollard, T. D., & Drubin, D. G. (2019). Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features. eLife, 8, e50749.

* Lou, H.-Y., Zhao, W., Li, X., Duan, L., Powers, A., Akamatsu, M., Santoro, F., McGuire, A.F., Cui, Y., Drubin, D.G., Cui, B. (2019). Membrane curvature underlies actin reorganization in response to nanoscale surface topography. PNAS, 201910166.

* Pedersen, R. T., & Drubin, D. G. (2019). Type I myosins anchor actin assembly to the plasma membrane during clathrin-mediated endocytosis. J Cell Biol, jcb-201810005.


* Schöneberg, J., Dambournet, D., Liu, T. L., Forster, R., Hockemeyer, D., Betzig, E., & Drubin, D. G. (2018). 4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell derived intestinal organoids. Molecular biology of the cell, mbc-E18.

*# Bergman, Z. J., Wong, J., Drubin, D. G., & Barnes, G. (2018). Microtubule dynamics regulation reconstituted in budding yeast lysates. J Cell Sci, jcs-219386.

* Dambournet, D., Sochacki, K. A., Cheng, A. T., Akamatsu, M., Taraska, J. W., Hockemeyer, D., & Drubin, D. G. (2018). Genome-edited human stem cells expressing fluorescently labeled endocytic markers allow quantitative analysis of clathrin-mediated endocytosis during differentiation. J Cell Biol, jcb-201710084.

* Liu, T. L., Upadhyayula, S., Milkie, D. E., Singh, V., Wang, K., Swinburne, I. A., …, Dambournet D., Forster, R., …, Hockemeyer D., Drubin D. G., …, Kirchhausen, T., & Betzig, E. (2018). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science, 360(6386), eaaq1392.

* Ibarlucea-Benitez, I., Ferro, L. S., Drubin, D. G., & Barnes, G. (2018). Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly. J Cell Biol, jcb-201708114.


* Sun, Y., Leong, N. T., Jiang, T., Tangara, A., Darzacq, X., & Drubin, D. G. (2017). Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo. eLife, 6.

* Zhao, W., Hanson, L., Lou, H.Y., Akamatsu, M., Chowdary, P., Santoro, F., Marks, J., Grassart, A., Drubin, D., Cui, Y. and Cui, B. (2017). Nanoscale manipulation of membrane curvature for probing endocytosis in live cells. Nature Nanotechnology, 12, 750–756.

^ Drubin, D. G., & Hyman, A. A. (2017). Stem cells: the new “model organism”. Molecular Biology of the Cell, 28(11), 1409-1411.

* Hassinger, J. E., Oster, G., Drubin, D. G., & Rangamani, P. (2017). Design principles for robust vesiculation in clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences, 114(7), E1118-E1127.
[MATLAB code here]

* Lu, R., & Drubin, D. G. (2017). Selection and stabilization of endocytic sites by Ede1, a yeast functional homologue of human Eps15. Molecular biology of the cell, 28(5), 567-575.


^ Drubin, D. G. (2016). The mismeasure of scientific research articles and why MBoC quickly embraced preprints. Molecular biology of the cell.

^ Schwarzbauer, J. E., Leader, W. M., & Drubin, D. G. (2016). Setting the bar for cell biology best practices. Molecular biology of the cell.

^ Lee, C. M., & Drubin, D. G. (2016). All together now: how and why scientific communities should develop best practice guidelines. Molecular biology of the cell.

^ Berg, J.M., Bhalla, N., Bourne, P.E., Chalfie, M., Drubin, D.G., Fraser, J.S., Greider, C.W., Hendricks, M., Jones, C., Kiley, R. and King, S. (2016). Preprints for the life sciences. Science, 352(6288), 899-901.

# Lu, R., Drubin, D. G., & Sun, Y. (2016). Clathrin-mediated endocytosis in budding yeast at a glance. J Cell Sci, 129(8), 1531-1536.

* Miao, Y., Han, X., Zheng, L., Xie, Y., Mu, Y., Yates III, J. R., & Drubin, D. G. (2016). Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nature communications, 7.


* Lewellyn, E. B., Pedersen, R. T., Hong, J., Lu, R., Morrison, H. M., & Drubin, D. G. (2015). An engineered minimal WASP-myosin fusion protein reveals essential functions for endocytosis. Developmental cell, 35(3), 281-294.

^ Drubin, D. G. (2015). Great science inspires us to tackle the issue of data reproducibility. Molecular biology of the cell.

* Hong, S. H., Cortesio, C. L., & Drubin, D. G. (2015). Machine-learning-based analysis in genome-edited cells reveals the efficiency of clathrin-mediated endocytosis. Cell reports, 12(12), 2121-2130.

* Sun, Y., Leong, N. T., Wong, T., & Drubin, D. G. (2015). A Pan1/End3/Sla1 complex links Arp2/3-mediated actin assembly to sites of clathrin-mediated endocytosis. Molecular biology of the cell, 26(21), 3841-3856.

* Krefman, N. I., Drubin, D. G., & Barnes, G. (2015). Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment. Molecular biology of the cell, 26(14), 2620-2639.

* Peng, Y., Moritz, M., Han, X., Giddings, T.H., Lyon, A., Kollman, J., Winey, M., Yates, J., Agard, D.A., Drubin, D.G. and Barnes, G. (2015). Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning. Molecular biology of the cell, 26(13), pp.2505-2518.

* Cortesio, C. L., Lewellyn, E. B., & Drubin, D. G. (2015). Control of lipid organization and actin assembly during clathrin-mediated endocytosis by the cytoplasmic tail of the rhomboid protein Rbd2. Molecular biology of the cell, 26(8), 1509-1522.

* Peng, Y., Grassart, A., Lu, R., Wong, C. C., Yates, J., Barnes, G., & Drubin, D. G. (2015). Casein kinase 1 promotes initiation of clathrin-mediated endocytosis. Developmental cell, 32(2), 231-240.


# Dambournet, D., Hong, S. H., Grassart, A., & Drubin, D. G. (2014). Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9. Methods Enzymol, 546, 139-160.

^ Drubin, D. G. (2014). Inspiration from inspirational cell biologists. Molecular biology of the cell.

* Pigula, A., Drubin, D. G., & Barnes, G. (2014). Regulation of mitotic spindle disassembly by an environmental stress-sensing pathway in budding yeast. Genetics, 198(3), 1043-1057.

^ Drubin, D. G. (2014). MBoC improves recognition of co–first authors. Molecular biology of the cell.

* Grassart, A., Cheng, A.T., Hong, S.H., Zhang, F., Zenzer, N., Feng, Y., Briner, D.M., Davis, G.D., Malkov, D. and Drubin, D.G. (2014). Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis. J Cell Biol, jcb-201403041.

* Weinberg, J. S., & Drubin, D. G. (2014). Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination. Current Biology, 24(9), 951-959.

# Michelot, A., & Drubin, D. G. (2014). Dissecting principles governing actin assembly using yeast extracts. Methods in enzymology, 540, 381-397.


* Miao, Y., Wong, C.C., Mennella, V., Michelot, A., Agard, D.A., Holt, L.J., Yates, J.R. and Drubin, D.G. (2013). Cell-cycle regulation of formin-mediated actin cable assembly. Proceedings of the National Academy of Sciences, 110(47), E4446-E4455.

* Zhao, H., Michelot, A., Koskela, E. V., Tkach, V., Stamou, D., Drubin, D. G., & Lappalainen, P. (2013). Membrane-sculpting BAR domains generate stable lipid microdomains. Cell reports, 4(6), 1213-1223.

* Faust, A. M. E., Wong, C. C., Yates III, J. R., Drubin, D. G., & Barnes, G. (2013). The FEAR Protein Slk19 Restricts Cdc14 Phosphatase to the Nucleus until the End of Anaphase, Regulating Its Participation in Mitotic Exit in Saccharomyces cerevisiae. PloS one, 8(9), e73194.

* Cormier, A., Drubin, D. G., & Barnes, G. (2013). Phosphorylation regulates kinase and microtubule binding activities of the budding yeast chromosomal passenger complex in vitro. Journal of Biological Chemistry, 288(32), 23203-23211.

^ Bertuzzi, S., & Drubin, D. G. (2013). No shortcuts for research assessment. Molecular biology of the cell.

* Spiess, M., de Craene, J.O., Michelot, A., Rinaldi, B., Huber, A., Drubin, D.G., Winsor, B. and Friant, S., 2013. Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis. PloS one, 8(4), p.e61147.

* Xin, X., Gfeller, D., Cheng, J., Tonikian, R., Sun, L., Guo, A., …, Drubin, D.G., …, & Bader, G.D. (2013). SH3 interactome conserves general function over specific form. Molecular systems biology, 9(1), 652.

* Buser, C., & Drubin, D. G. (2013). Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling. Microscopy and Microanalysis, 19(2), 381-392.

* Michelot, A., Grassart, A., Okreglak, V., Costanzo, M., Boone, C., & Drubin, D. G. (2013). Actin filament elongation in Arp2/3-derived networks is controlled by three distinct mechanisms. Developmental cell, 24(2), 182-195.


* Sun, Y., & Drubin, D. G. (2012). The functions of anionic phospholipids during clathrin-mediated endocytosis site initiation and vesicle formation. J Cell Sci, 125(24), 6157-6165.

* Mattiazzi, M., Sun, Y., Wolinski, H., Bavdek, A., Petan, T., Anderluh, G., Kohlwein, S.D., Drubin, D.G., Križaj, I. and Petrovič, U. (2012). A neurotoxic phospholipase A2 impairs yeast amphiphysin activity and reduces endocytosis. PloS one, 7(7), e40931.

* Cheng, J., Grassart, A., & Drubin, D. G. (2012). Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis. Molecular biology of the cell, 23(15), 2891-2904.

* Sun, Y., Miao, Y., Yamane, Y., Zhang, C., Shokat, K.M., Takematsu, H., Kozutsumi, Y. and Drubin, D.G. (2012). Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Molecular biology of the cell, 23(12), 2388-2398.

^ Drubin, D. G., & Kellogg, D. R. (2012). English as the universal language of science: opportunities and challenges. Molecular biology of the cell.

^ Sun, Y., & Drubin, D. G. (2012). Pik1-ing clathrin adaptors. Nature cell biology, 14(3), 231.

* Carroll, S. Y., Stimpson, H. E., Weinberg, J., Toret, C. P., Sun, Y., & Drubin, D. G. (2012). Analysis of yeast endocytic site formation and maturation through a regulatory transition point. Molecular biology of the cell, 23(4), 657-668.

* Woodruff, J. B., Drubin, D. G., & Barnes, G. (2012). Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle. Molecular biology of the cell, 23(2), 258-267.

# Weinberg, J., & Drubin, D. G. (2012). Clathrin-mediated endocytosis in budding yeast. Trends in cell biology, 22(1), 1-13.


^ Kellogg, D. R., & Drubin, D. G. (2011). MBoC 2011: same values, improved feng shui. Molecular biology of the cell.

* Kishimoto, T., Sun, Y., Buser, C., Liu, J., Michelot, A., & Drubin, D. G. (2011). Determinants of endocytic membrane geometry, stability, and scission. Proceedings of the National Academy of Sciences, 108(44), E979-E988.

# Michelot, A., & Drubin, D. G. (2011). Building distinct actin filament networks in a common cytoplasm. Current Biology, 21(14), R560-R569.

* Nakajima, Y., Cormier, A., Tyers, R. G., Pigula, A., Peng, Y., Drubin, D. G., & Barnes, G. (2011). Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC–spindle interaction to ensure proper microtubule dynamics. The Journal of cell biology, 194(1), 137-153.

* Peng, Y., Wong, C.C., Nakajima, Y., Tyers, R.G., Sarkeshik, A.S., Yates, J., Drubin, D.G. and Barnes, G. (2011). Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation. Molecular biology of the cell, 22(15), pp.2680-2689.

* Uchida, M., Sun, Y., McDermott, G., Knoechel, C., Le Gros, M.A., Parkinson, D., Drubin, D.G. and Larabell, C.A., 2011. Quantitative analysis of yeast internal architecture using soft X‐ray tomography. Yeast, 28(3), pp.227-236.

^ Drubin, D. G. (2011). Any jackass can trash a manuscript, but it takes good scholarship to create one (how MBoC promotes civil and constructive peer review). Molecular biology of the cell.

* Doyon, J. B., Zeitler, B., Cheng, J., Cheng, A. T., Cherone, J. M., Santiago, Y., … & Drubin, D.G. (2011). Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells. Nature cell biology, 13(3), 331.

* Ramey, V.H., Wang, H.W., Nakajima, Y., Wong, A., Liu, J., Drubin, D.G., Barnes, G. and Nogales, E. (2011). The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. Molecular biology of the cell, 22(4), 457-466.

* Layton, A. T., Savage, N. S., Howell, A. S., Carroll, S. Y., Drubin, D. G., & Lew, D. J. (2011). Modeling vesicle traffic reveals unexpected consequences for Cdc42p-mediated polarity establishment. Current Biology, 21(3), 184-194.


* Woodruff, J. B., Drubin, D. G., & Barnes, G. (2010). Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8. The Journal of cell biology, 191(4), 795-808.

^ Kellogg, D. R., & Drubin, D. G. (2010). The 50th Anniversary of the ASCB: Celebrating by Looking Forward. Molecular biology of the cell.

* Michelot, A., Costanzo, M., Sarkeshik, A., Boone, C., Yates, J. R., & Drubin, D. G. (2010). Reconstitution and protein composition analysis of endocytic actin patches. Current Biology, 20(21), 1890-1899.

^ Drubin, D. G., & Oster, G. (2010). Experimentalist meets theoretician: a tale of two scientific cultures. Molecular biology of the cell, 21(13), 2099-2101.

* Okreglak, V., & Drubin, D. G. (2010). Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway. The Journal of cell biology, jcb-200909176.

^ Drubin, D. G. (2010). Are you being served?. Molecular biology of the cell.

# Liu, J., Sun, Y., Oster, G. F., & Drubin, D. G. (2010). Mechanochemical crosstalk during endocytic vesicle formation. Current opinion in cell biology, 22(1), 36-43.