Paper by Joh on analysis of lattice light sheet imaging data now published online in MBoC

Congratulations to Joh and former lab member Daphne on their paper “4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell derived intestinal organoids“, now published online in Molecular biology of the cell.

A new level of 4D tissue cell biology is unlocked as recent advances from three fields come together. (A) Endogenous protein tagging using genome editing (i), stem cell biology (ii) and 3D tissue/organoid culture (iii), (B) 4D non-invasive advanced fluorescent imaging with the lattice light-sheet microscope with adaptive optics (AO-LLSM, left: full view of the microscope, right: focus on the characteristic objective arrangement) and (C) advances and software in big data image analytics. (Right) Combination of these elements allows unprecedented quantitative analysis of subcellular events within live tissues in 4D.

Paper by Zane and Jonathan on microtubule dynamics accepted by JCS

Congratulations to Zane and Jonathan on their paper “Microtubule dynamics regulation reconstituted in budding yeast lysates“, now accepted and online in the Journal of Cell Science.

Recreation of Microtubule Dynamics in Budding Yeast Lysate. A reaction chamber is prepared by adhering GMPCPP-stabilized, rhodamine-labeled porcine microtubule seeds (red) to a glass coverslip through a biotin (black stems)-neutravidin (orange ovals) system. Whole-cell lysate from GFP-tubulin (green) expressing yeast strains is flowed into the chamber and incubated to allow for the polymerization of microtubules. Growth and dynamics of single microtubules is observed by TIRF microscopy in the context of other soluble proteins found in the cell (black shapes).

Goodbye Daphne, Itziar and Emily!

The Drubin/Barnes lab would like to send our fondest farewells to Daphne, Itziar and Emily who are moving on to exciting new positions!

Daphne Dambournet is now working as a Cell line engineer at Memphis Meats working to grow sustainable cultured meat.

Itziar Ibarlucea has taken a position as a post-doctoral associate in the laboratory of Jeffrey Ravetch at The Rockefeller University.

Emily Stoops is now working as a Scientist at Calico where she is working to combat aging.

Paper by Yidi Sun now published in eLife

Congratulations to Yidi, her former and current undergraduates Niki and Tommy, and collaborators in the Darzacq Lab, on their new paper “Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo” now published at eLife!

Switch-like activation of the Arp2/3 complex mediated by SH3 domain-PRM mediated interactions during yeast CME.

Goodbye Jessica!

The Drubin/Barnes lab celebrated post-doc Jessica Marks moving on to her new position as a AAAS Fellow. We wish her all the best in Washington, DC!

Celebration at Brazil Cafe as Jessica moves to a new position a AAAS Fellow in D.C.