More media will be posted shortly.
Above: The budding yeast Saccharomyces cerevisiae containing GFP-tagged versions of the proteins Myo1 (myosin) and Tub1 (tubulin). This live-cell assay allows us to investigate the temporal relationship between the disassembly of the mitotic spindle (visualized with Tub1-GFP) and the contraction of the cytokinetic ring (visualized with Myo1-GFP). In wild-type cells, the mitotic spindle depolymerizes before the cytokinetic ring contracts. In several spindle disassembly mutants that our lab has characterized, spindle disassembly is delayed until after onset of cytokinetic ring contraction. [Video from Anthony Cormier.]
Above: Saccharomyces cerevisiae containing Ras2-GFP and Spc42-mCherry. This strain allows us to visualize the spindle pole bodies (SPBs, visualized with Spc42-mCherry) through mitosis, while also observing membrane contraction at the budneck (visualized with Ras2-GFP). [Image from Anthony Cormier.]