Model for Myo5 function in anchoring actin assembly to the PM at endocytic sites. (A) Myo5 (yellow bananas) restricts activation of the Arp2/3 complex (gray avocados) by the WASP complex (blue widgets) to a discrete location, generating an actin array that grows predominantly in the same direction to generate force. (B) Absent this critical linkage, Arp2/3 activators splinter off of the PM, leading to Arp2/3 complex activation throughout the actin network. Delocalized Arp2/3 complex activation results in disordered actin arrays that fail to produce force. In the most catastrophic cases, the Arp2/3 complex and its activators pull away from the PM completely to form cytoplasmic actin comets (lower left of zoom).

A new level of 4D tissue cell biology is unlocked as recent advances from three fields come together. (A) Endogenous protein tagging using genome editing (i), stem cell biology (ii) and 3D tissue/organoid culture (iii), (B) 4D non-invasive advanced fluorescent imaging with the lattice light-sheet microscope with adaptive optics (AO-LLSM, left: full view of the microscope, right: focus on the characteristic objective arrangement) and (C) advances and software in big data image analytics. (Right) Combination of these elements allows unprecedented quantitative analysis of subcellular events within live tissues in 4D.

Recreation of Microtubule Dynamics in Budding Yeast Lysate. A reaction chamber is prepared by adhering GMPCPP-stabilized, rhodamine-labeled porcine microtubule seeds (red) to a glass coverslip through a biotin (black stems)-neutravidin (orange ovals) system. Whole-cell lysate from GFP-tubulin (green) expressing yeast strains is flowed into the chamber and incubated to allow for the polymerization of microtubules. Growth and dynamics of single microtubules is observed by TIRF microscopy in the context of other soluble proteins found in the cell (black shapes).

Endocytic dynamics in three isogenic cell types.

The CPC cannot concentrate at the spindle midzone in the absence of Kip1 and Kip3.