For Journal Club on August 6, Nate Krefman presented the following paper:
Centromere-like regions in the budding yeast genome. Lefrançois P, Auerbach RK, Yellman CM, Roeder GS, Snyder M. PLoS Genet. 2013;9(1):e1003209. PMID: 23349633.
CLRs confer centromere function on plasmids and chromosomes.
For our next Journal Club, our summer undergraduate researcher Josh Johnson will present the following paper:
Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49. PMID: 21844210.
Centromeres (CB-mCherry) and chromosomes (YFP emission) for a single time point. (bottom) Color-coded YFP/CFP emission ratio at different time points. The timestamp (minutes and seconds) is relative to ZM washout at t = 0. Bar, 5 µm.