Tag Archives: membranes

Journal Club on Monday, June 17

For our Journal Club on June 17, Jessica Marks will present the following paper:

Iwamoto PNAS 2013

Hypothetical energy profiles for the closed and open transition states.

Amphipathic antenna of an inward rectifier K+ channel responds to changes in the inner membrane leaflet. Iwamoto M, Oiki S. Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):749-54. PMID: 23267068.

Journal Club on Monday, June 3

For our Journal Club on June 3, Daphné Dambournet presented the following paper:

Divergent modes for cargo-mediated control of clathrin-coated pit dynamics. Soohoo AL, Puthenveedu MA. Mol Biol Cell. 2013 Jun;24(11):1725-34. PMID: 23536704.

Soohoo MBoC 2013

Model for divergent modes of cargo-mediated control of endocytosis by signaling receptors.

Journal Club on Monday, May 13

For our Journal Club on May 13, Christa Cortesio presented the following paper:

ER-to-plasma membrane tethering proteins regulate cell signaling and ER morphology. Manford AG, Stefan CJ, Yuan HL, Macgurn JA, Emr SD. Dev Cell. 2012 Dec 11;23(6):1129-40. PMID: 23237950.

Manford et al Dev Cell 2012

Localization of the ER marker Sec61-GFP in wild-type and Δtether cells (ist2Δ, scs2/22Δ, and tcb1/2/3Δ).

Journal Club on Monday, April 29

For our Journal Club on April 29, Sun Hae Hong presented the following paper:

The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Miller SE, Sahlender DA, Graham SC, Höning S, Robinson MS, Peden AA, Owen DJ. Cell. 2011 Nov 23;147(5):1118-31. PMID: 22118466

Schematic representation of the model of VAMP8 trafficking from the plasma membrane.

Journal Club on Thursday, April 18

For our Journal Club on April 18, Akemi Kunibe presented the following paper:

Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Lackner LL, Ping H, Graef M, Murley A, Nunnari J.  Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):E458-67. PMID: 23341591.

The ER is a component of the Num1 mitochondria–cell cortex tether.

Journal Club on Monday, March 17

For our Journal Club on March 17, Rebecca Lu will present the following paper:

Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism. Jose M, Tollis S, Nair D, Sibarita JB, McCusker D. J Cell Biol. 2013 Feb 18;200(4):407-18. PMID: 23401000

Schematics illustrating the mathematical model. Shown are the Cdc42 autoamplification module (A), the complete endocytosis module (B), the exocytosis module (C), and a legend for graphics (D).

Journal Club on Monday, January 7

For our Journal Club on January 7, Yidi Sun presented the following paper:

PtdIns4P synthesis by PI4KIIIα at the plasma membrane and its impact on plasma membrane identity.  Nakatsu F, Baskin JM, Chung J, Tanner LB, Shui G, Lee SY, Pirruccello M, Hao M, Ingolia NT, Wenk MR, De Camilli P.  J Cell Biol. 2012 Dec 10;199(6):1003-16.  PMID:  23229899.

Congratulations to Yidi Sun on her new paper!

Yidi Sun‘s new paper is out now as an electronic publication ahead of print in the Journal of Cell Science.  Congratulations to Yidi on her great work!  The abstract is below.  The PDF can be downloaded from JCS here.

The functions of anionic phospholipids during clathrin-mediated endocytosis site initiation and vesicle formation. Sun Y, Drubin DG. J Cell Sci. 2012 Oct 24. PMID: 23097040.

Abstract

Anionic phospholipids PI(4,5)P(2) and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P(2) and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Live-cell imaging of endocytic protein dynamics in an mss4(ts) mutant, which has severely reduced PI(4,5)P(2) levels, revealed that PI(4,5)P(2) is required for endocytic membrane invagination but is less important for endocytic site initiation. We also demonstrated that in various deletion mutants of genes encoding components of the Rcy1-Ypt31/32 GTPase pathway, endocytic proteins dynamically assemble not only on the plasma membrane but also on intracellular membrane compartments, which are likely derived from early endosomes. In rcy1Δ cells, fluorescent biosensors indicated that PI(4,5)P(2) only localized to the plasma membrane while PS localized to both the plasma membrane and intracellular membranes. Furthermore, we found that polarized endocytic patch establishment is defective in the PS-deficient cho1Δ mutant. We propose that PS is important for directing endocytic proteins to the plasma membrane and that PI(4,5)P(2) is required to facilitate endocytic membrane invagination.

Journal Club on Monday, July 09

For our next Journal Club, Adrianne Pigula will present the following paper:

A link between mitotic entry and membrane growth suggests a novel model for cell size control.  Anastasia SD, Nguyen DL, Thai V, Meloy M, MacDonough T, Kellogg DR.  J Cell Biol. 2012 Apr 2;197(1):89-104. Epub 2012 Mar 26.  PMID: 22451696.