Tag Archives: particle tracking

Microtubules and Mitosis Journal Club on Monday, August 26

For Microtubules and Mitosis Journal Club on August 26, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Itziar Ibarlucea:

Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Cell. 2013 Jul 18;154(2):377-90. PMID: 23870126.

Subramanian Cell 2013

graphical abstract of the paper

Microtubules and Mitosis Journal Club on Monday, June 24

For Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Stu Howes:

Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Zanic M, Widlund PO, Hyman AA, Howard J. Nat Cell Biol. 2013 Jun;15(6):688-93. PMID: 23666085.

Zanic NCB 2013

Reaction scheme for an enzymatic reaction with two non-essential activators. The microtubule end is viewed as an enzyme that catalyses the reaction: tubulin in solution (substrate)→tubulin in the polymer (product) by providing a much more efficient pathway than direct incorporation into the lattice. XMAP215 and EB1 are viewed as two non-essential enzyme activators that accelerate the net rate of addition at the end. Filled arrows represent the flux of polymerization through a particular vertex. M, microtubule end; T, free tubulin; X, XMAP215; E, EB1.

Journal Club on Monday, Feb. 7

For our Journal Club on February 7, Yansong Miao will present the following paper:

Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging. Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL. Science. 2012 Jun 1;336(6085):1164-8. doi: 10.1126/science.1218062. PMID: 22654058

Proposed ‘rocket launcher’ model for mDia1-APC collaboration during actin filament assembly

Journal Club on Monday, April 2

For our next Journal Club, Sun Hae Hong will present the following paper:

Influence of synaptic vesicle position on release probability and exocytotic fusion mode.  Park H, Li Y, Tsien RW.  Science.  2012 Mar 16;335(6074):1362-6.  PMID: 22345401.

 

Journal Club on Monday, March 05

For our last Journal Club, Itziar Benitez-Ibarlucea presented the following paper:

Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains. DeWitt MA, Chang AY, Combs PA, Yildiz A. Science. 2012 Jan 13;335(6065):221-5. PMID: 22157083