For Microtubules and Mitosis Journal Club on August 26, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Itziar Ibarlucea:
Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Cell. 2013 Jul 18;154(2):377-90. PMID: 23870126.
graphical abstract of the paper
For Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Stu Howes:
Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Zanic M, Widlund PO, Hyman AA, Howard J. Nat Cell Biol. 2013 Jun;15(6):688-93. PMID: 23666085.
Reaction scheme for an enzymatic reaction with two non-essential activators. The microtubule end is viewed as an enzyme that catalyses the reaction: tubulin in solution (substrate)→tubulin in the polymer (product) by providing a much more efficient pathway than direct incorporation into the lattice. XMAP215 and EB1 are viewed as two non-essential enzyme activators that accelerate the net rate of addition at the end. Filled arrows represent the flux of polymerization through a particular vertex. M, microtubule end; T, free tubulin; X, XMAP215; E, EB1.
For our new Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab discussed the following paper, selected by Nate Krefman:
Estimating the microtubule GTP cap size in vivo. Seetapun D, Castle BT, McIntyre AJ, Tran PT, Odde DJ. Curr Biol. 2012 Sep 25;22(18):1681-7. PMID: 22902755
Example EB1 comet from an EB1-EGFP LLCPK1 cell and the corresponding fluorescence intensity (FI) linescan, blue line.