For Microtubules and Mitosis Journal Club on August 26, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Itziar Ibarlucea:
Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Cell. 2013 Jul 18;154(2):377-90. PMID: 23870126.
graphical abstract of the paper
For Journal Club on August 6, Nate Krefman presented the following paper:
Centromere-like regions in the budding yeast genome. Lefrançois P, Auerbach RK, Yellman CM, Roeder GS, Snyder M. PLoS Genet. 2013;9(1):e1003209. PMID: 23349633.
CLRs confer centromere function on plasmids and chromosomes.
For Journal Club on August 6, Eric Lewellyn presented the following paper:
Actin filament severing by cofilin dismantles actin patches and produces mother filaments for new patches. Chen Q, Pollard TD. Curr Biol. 2013 Jul 8;23(13):1154-62. PMID: 23727096.
“Sever, diffuse and trigger” model for actin filament turnover in actin patches. The 7 steps (numbers next to the arrows) are (1) clathrin coated pits bind adaptor proteins End4p and Pan1p, (2) short, diffusing actin filaments bind to End4p and Pan1p associated with coated pits, and (3) Arp2/3 complex interacts with these mother filaments and nucleation promoting factors to (4) initiate branching nucleation of actin filaments that promote elongation of the endocytic tubule. (5) After abscission of the vesicle, (6) cofilin severs actin filaments to generate a pool of short, diffusing actin filaments, some of which return to the cycle at step 2.
For Journal Club on July 22, Connie Peng presented the following paper:
Mps1 and Ipl1/Aurora B act sequentially to correctly orient chromosomes on the meiotic spindle of budding yeast. Meyer RE, Kim S, Obeso D, Straight PD, Winey M, Dawson DS. Science. 2013 Mar 1;339(6123):1071-4. PMID: 23371552
Meyer Science 2013