Congratulations to Rebecca Lu on her paper, “Selection and stabilization of endocytic sites by Ede1, a yeast functional homolog of the human Eps15“, now in press in Molecular Biology of the Cell.
Congrats Akemi and Rebecca!
Congratulations to Akemi and Rebecca, the two newest PhD’s out of the Drubin/Barnes Lab!
Congratulations Niki and Gloria!
New Cal Alumni! We are all so proud of you and happy for you! Go Bears!! – 5/18/2016
Welcome to the lab, Bob!
Port tasting after David and Georjana’s trip to Portugal!
After hosting visiting scholar Prof. Sandra Paiva and then her student Rosana, David and Georjana decided to take a trip to Portugal to visit. Upon their return, they treated the lab to a port and cheese tasting.
Happy Halloween 2014 from the Drubin/Barnes Lab!
Enjoy a video of the disco pumpkin made by Yidi Sun at the Drubin/Barnes, Koshland, Brar, Ünal, and Ingolia Labs (Barker Hall) pumpkin carving party to celebrate Halloween 2014:
You can now follow David Drubin on Twitter @DavidGDrubin!
David Drubin, in his capacity as Editor-in-Chief of Molecular Biology of the Cell, has just joined Twitter. You can follow his tweets by clicking the link below:
Congratulations to Yansong Miao on his new paper!
Yansong Miao‘s new paper is out now at The Proceedings of the National Academy of Sciences. Congratulations to Yansong on his great work! The abstract is below. The PDF can be downloaded from PNAS here.
Cell-cycle regulation of formin-mediated actin cable assembly. Miao Y, Wong CC, Mennella V, Michelot A, Agard DA, Holt LJ, Yates JR 3rd, Drubin DG. Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4446-E4455. Epub 2013 Oct 16. PMID: 24133141
Abstract
Assembly of appropriately oriented actin cables nucleated by formin proteins is necessary for many biological processes in diverse eukaryotes. However, compared with knowledge of how nucleation of dendritic actin filament arrays by the actin-related protein-2/3 complex is regulated, the in vivo regulatory mechanisms for actin cable formation are less clear. To gain insights into mechanisms for regulating actin cable assembly, we reconstituted the assembly process in vitro by introducing microspheres functionalized with the C terminus of the budding yeast formin Bni1 into extracts prepared from yeast cells at different cell-cycle stages. EM studies showed that unbranched actin filament bundles were reconstituted successfully in the yeast extracts. Only extracts enriched in the mitotic cyclin Clb2 were competent for actin cable assembly, and cyclin-dependent kinase 1 activity was indispensible. Cyclin-dependent kinase 1 activity also was found to regulate cable assembly in vivo. Here we present evidence that formin cell-cycle regulation is conserved in vertebrates. The use of the cable-reconstitution system to test roles for the key actin-binding proteins tropomyosin, capping protein, and cofilin provided important insights into assembly regulation. Furthermore, using mass spectrometry, we identified components of the actin cables formed in yeast extracts, providing the basis for comprehensive understanding of cable assembly and regulation.
Happy Halloween from the Drubin/Barnes Lab!!
Microtubules and Mitosis Journal Club on Monday, August 26
For Microtubules and Mitosis Journal Club on August 26, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Itziar Ibarlucea:
Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Cell. 2013 Jul 18;154(2):377-90. PMID: 23870126.