For Microtubules and Mitosis Journal Club on August 26, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Itziar Ibarlucea:
Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Cell. 2013 Jul 18;154(2):377-90. PMID: 23870126.
graphical abstract of the paper
For Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab will discuss the following paper, selected by Stu Howes:
Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Zanic M, Widlund PO, Hyman AA, Howard J. Nat Cell Biol. 2013 Jun;15(6):688-93. PMID: 23666085.
Reaction scheme for an enzymatic reaction with two non-essential activators. The microtubule end is viewed as an enzyme that catalyses the reaction: tubulin in solution (substrate)→tubulin in the polymer (product) by providing a much more efficient pathway than direct incorporation into the lattice. XMAP215 and EB1 are viewed as two non-essential enzyme activators that accelerate the net rate of addition at the end. Filled arrows represent the flux of polymerization through a particular vertex. M, microtubule end; T, free tubulin; X, XMAP215; E, EB1.
For our Journal Club on June 17, Jessica Marks will present the following paper:
Hypothetical energy profiles for the closed and open transition states.
Amphipathic antenna of an inward rectifier K+ channel responds to changes in the inner membrane leaflet. Iwamoto M, Oiki S. Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):749-54. PMID: 23267068.
For our new Microtubules and Mitosis Journal Club on April 25, members of the Drubin/Barnes Lab, Eva Nogales Lab, and Ahmet Yildiz Lab discussed the following paper, selected by Nate Krefman:
Estimating the microtubule GTP cap size in vivo. Seetapun D, Castle BT, McIntyre AJ, Tran PT, Odde DJ. Curr Biol. 2012 Sep 25;22(18):1681-7. PMID: 22902755
Example EB1 comet from an EB1-EGFP LLCPK1 cell and the corresponding fluorescence intensity (FI) linescan, blue line.
For our Journal Club on March 17, Rebecca Lu will present the following paper:
Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism. Jose M, Tollis S, Nair D, Sibarita JB, McCusker D. J Cell Biol. 2013 Feb 18;200(4):407-18. PMID: 23401000
Schematics illustrating the mathematical model. Shown are the Cdc42 autoamplification module (A), the complete endocytosis module (B), the exocytosis module (C), and a legend for graphics (D).
For our Journal Club on February 7, Yansong Miao will present the following paper:
Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging. Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL. Science. 2012 Jun 1;336(6085):1164-8. doi: 10.1126/science.1218062. PMID: 22654058
Proposed ‘rocket launcher’ model for mDia1-APC collaboration during actin filament assembly
For our next Journal Club, Padmini Rangamani will present the following paper:
Phase transitions in the assembly of multivalent signalling proteins. Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Nature. 2012. PMID: 22398450
Phase transition correlates to biochemical activity transition in the nephrin/Nck/N-WASP system
For our next Journal Club, our summer undergraduate researcher Josh Johnson will present the following paper:
Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49. PMID: 21844210.
Centromeres (CB-mCherry) and chromosomes (YFP emission) for a single time point. (bottom) Color-coded YFP/CFP emission ratio at different time points. The timestamp (minutes and seconds) is relative to ZM washout at t = 0. Bar, 5 µm.
For our next Journal Club, Sun Hae Hong will present the following paper:
Influence of synaptic vesicle position on release probability and exocytotic fusion mode. Park H, Li Y, Tsien RW. Science. 2012 Mar 16;335(6074):1362-6. PMID: 22345401.
For our last Journal Club, Itziar Benitez-Ibarlucea presented the following paper:
Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains. DeWitt MA, Chang AY, Combs PA, Yildiz A. Science. 2012 Jan 13;335(6065):221-5. PMID: 22157083